|
Primary Antibodies
Nanos3 Antibody
Background
Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects. This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.
Additional Names
Nanos3 (CT), Nanos homolog 3
|
Description
Left: Western blot analysis of Nanos3 in human brain tissue lysate with Nanos3 antibody at 2 μg/ml.
Below: Immunohistochemistry of Nanos3 in human brain tissue with Nanos3 antibody at 2.5 μg/ml.
|
|
Other Product Images
|
Source
Nanos3 antibody was raised against a 16 amino acid peptide near the carboxy terminus of the human Nanos3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Nanos3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Nanos3 antibody can be used for detection of Nanos3 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4653P - Nanos3 Peptide
Storage
Nanos3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
41688563
Protein Accession Number
P60323
Short Description
(CT) Nanos homolog 3
This product belongs to the following categories:
Related Products
References- Lehmann R and Nusslein-Volhard C. The maternal gene nanos has a central role in posterior pattern formation of the drosophila embryo. Development 1991; 112:679-91.
- Tsuda M, Sasaoka Y, Kiso M, et al. Conserved role of nanos proteins in germ cell development. Science 2003; 301:1239-41.
- Sato K, Hayashi Y, Ninomiya Y, et al. Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos. Proc. Natl. Acad. Sci. USA 2007; 104:7455-60.
- Haraguchi S, Tsuda M, Kitajima S, et al. Nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development. Mech. Dev. 2003; 120:721-31.
Datasheet 08-03W
|
|
