|
Primary Antibodies
Nanos1 Antibody
Background
Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.
Additional Names
Nanos1 (NT), Nanos homolog 1, NOS1
|
Description
Left: Western blot analysis of Nanos1 in SK-N-SH cell lysate with Nanos1 antibody at 1 μg/ml in (A) the presence and (B) the absence of blocking peptide.
Below: Immunohistochemistry of Nanos1 in human brain tissue with Nanos1 antibody at 2.5 μg/ml
|
|
Other Product Images
|
Source
Nanos1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human Nanos1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Nanos1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Nanos1 antibody can be used for detection of Nanos1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4683P - Nanos1 Peptide
Storage
Nanos1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1220 - SK-N-SH Cell Lysate
Species Reactivity
H
Protein GI Number
41688589
Protein Accession Number
Q8WY41
Short Description
(NT) Nanos homolog 1
This product belongs to the following categories:
Related Products
References- Jaruleska J, Kotecki M, Kusz K, et al. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. Dev. Genes Evol. 2003; 213:120-6.
- Tsuda M, Sasaoka Y, Kiso M, et al. Conserved role of nanos proteins in germ cell development. Science 2003; 301:1239-41.
- Haraguchi S, Tsuda M, Kitajima S, et al. Nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development. Mech. Dev. 2003; 120:721-31.
- Strumane K, Bonnomet A, Stove A, et al. E-cadherin regulates human Nanos1, which interacts with p120ctn and induces tumor cell migration and invasion. Cancer Res. 2006; 66:10007-15.
Datasheet 08-03W
|
|
