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Primary Antibodies
NOD2 Antibody
Background
Apaf-1 and NOD1 are members of a new family (1), which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2 (2). NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn’s disease (3,4) that is a main type of chronic inflammatory bowel disease.
Additional Names
NOD2 (NT), Caspase recruitment domain 15, CARD15
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Description
Left: Western blot analysis of NOD2 in Jurkat cell lysate with NOD2 antibody at (A) 1, (B) 2 and (C) 4 μg/ml.
Below: Immunocytochemistry of NOD2 in Jurkat cells with NOD2 antibody at 5 μg/ml.
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Other Product Images
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Source
Rabbit NOD2 polyclonal antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2.
Purification
Immunoaffinity chromatography purified IgG
Clonality / Clone
This is a polyclonal antibody.
Host
NOD2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human NOD2 (N-Terminus) Peptide (Cat. No. 2511P)
Application
NOD2 antibody can be used for detection of Noxa by Western blot at 2 to 4 μg/ml. NOD2 has no cross-reaction with NOD1.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2511P - NOD2 Peptide
Storage
NOD2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein GI Number
20137973
Protein Accession Number
Q9HC29
Short Description
(NT) A new CARD-containing protein
This product belongs to the following categories:
Related Products
References- Inohara N, Koseki T, del Peso L, et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. J. Biol. Chem. 1999; 274:14560-7.
- Ogura Y, Inohara N, Benito A, et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-κB. J. Biol. Chem. 2001; 276:4812-8.
- Hugot JP, Chamaillard M, et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease. Nature 2001; 411:599-603.
- Ogura Y, Bonen DK, Inohara N, et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature 2001; 411:603-6.
Datasheet 08-02W
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