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Primary Antibodies
NOD2 Antibody
Background
NOD2 Antibody.
Additional Names
CARD15, caspase recruitment domain family, member 15, ACUG, BLAU, CD, IBD1, NOD2, NOD2B, PSORAS1, LRR-containing protein, NOD2 protein, caspase recruitment domain protein 15, inflammatory bowel disease protein 1, nucleotide-binding oligomerization domain 2;
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Description
Left: Western blot analysis of NOD2 in human Peripheral Blood Mononucleocyte lysate (35μg protein in RIPA buffer) using NOD2 antibody (0.1μg/mL).
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Source
NOD2 antibody was raised against a synthetic peptide near an internal region of NOD2.
Purification
NOD2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
NOD2 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approx 100kDa band observed in lysates of human peripheral blood mononucleocytes (calculated MW of 115kDa according to NP_071445.1). Recommended concentration: 0.1-0.3μg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.
Tested Application
E, WB
Buffer
NOD2 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H, M, R
Protein Accession Number
NP_071445.1
Short Description
(IN) CARD15 / NOD2
This product belongs to the following categories:
Related Products
References- Keshav S. Paneth cells: leukocyte-like mediators of innate immunity in the intestine. J Leukoc Biol. 2006 Jun 22;
Datasheet 08-01W
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