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Primary Antibodies
NMDA Antibody
Background
The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
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Description
Left: Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.
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Purification
Serum
Clonality / Clone
This is a polyclonal antibody.
Host
NMDA antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Fusion protein from the C-terminus region of the NR2A subunit of rat NMDA receptor.
Application
The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.
Tested Application
WB
Buffer
NMDA antibody is supplied as neat serum
Storage
NMDA antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Species Reactivity
H, M, R
Short Description
(CT) NMDA NR2A Subunit Pan-Specific
This product belongs to the following categories:
Related Products
References- Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
- Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
- Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
Datasheet 0803-W
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