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Primary Antibodies
NK3R Antibody
Background
The tachykinins are a family of small peptides that include the neurotransmitters substance P, neurokinin A, and neurokinin B, which can act on three related but distinct seven transmembrane G-proteins coupled receptors, albeit at different concentrations. The NK-3 receptor (NK3R) has greatest affinity for neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system. It is likely these signals lead to the release of vasopressin and oxytocin into the circulation. NK3R may be involved in learning and memory as mice lacking this gene expressed cognitive deficits compared to normal mice. Although it has been suggested that NK3R plays a role in the regulation of vagal afferent relay neurons, it is likely that these receptors are activated by substance P or neurokinin A, as the airway nerves do not express neurokinin B. NK3R antibody does not recognize NK1R or NK2R.
Additional Names
NK3R (IN), Neurokinin B receptor, NK-3 receptor, NK-3R, Tachykinin receptor 3, TACR3
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Description
Left: Western blot analysis of NK3R in RAW264.7 cell lysate with NK3R antibody at (A) 0.5 and (B) 2 μg/ml.
Below: Immunohistochemistry of NK3R in human brain tissue with NK3R antibody at 5 μg/ml.
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Other Product Images
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Source
NK3R antibody was raised against an 18 amino acid peptide from near the center of human NK3R.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
NK3R antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
NK3R antibody can be used for detection of NK3R by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4255P - NK3R Peptide
Storage
NK3R antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1283 - RAW264.7 Cell Lysate
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M
Protein GI Number
128364
Protein Accession Number
P29371
Short Description
(IN) Neurokinin B receptor
This product belongs to the following categories:
Related Products
References- Maggi CA. The mammalian tachykinin receptors. Gen. Pharmacol. 1995; 26:911-44.
- Ding Y-Q, Shi J, Su L-Y, et al. Receptor (NK3)-containing neurons in the paraventricular and supraoptic nuclei of the rat hypothalamus synthesize vasopressin and express fos following intravenous injection of hypotonic saline. Neurosci. 1999; 91:1077-85.
- Nakajima Y, Tsuchida K, Negishi M, et al. Direct linkage of three tachykinin receptors to stimulation of both phosphatidylinositol hydrolysis and cyclic AMP cascades in transfected Chinese hamster ovary cells. J. Biol. Chem. 1992; 267:2437-42.
- Haley GE and Flynn FW. Tachykinin NK3 receptor contribution to systemic release of vasopressin and oxytocin in response to osmotic and hypotensive challenge. Am. J. Regul. Integr. Comp. Physiol. 2007; 293:R931-7.
Datasheet 08-02W
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