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Primary Antibodies
NIK Antibody
Background
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase) . NIK is an activator of IκB kinase α and β (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
Additional Names
NIK (CT), NIK
Source
NIK antibody was raised against a peptide corresponding to amino acids 931 to 947 of human NIK .
Purification
Antibody is DEAE purified
Clonality / Clone
This is a polyclonal antibody.
Host
NIK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human NIK (C-Terminus) Peptide (Cat. No. 1129P)
Application
NIK antibody can be used for detection of NIK by Western blot.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1129P - NIK Peptide
Storage
NIK antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1210 - 293 Cell Lysate
Species Reactivity
H
Protein GI Number
92090612
Protein Accession Number
Q99558
Short Description
(CT) NF-κB inducing kinase
This product belongs to the following categories:
Related Products
References- Malinin NL, Boldin MP, Kovalenko AV, Wallach . MAP3K-related kinase involved in NF-κB induction by TNF, CD95 and IL-1. Nature 1997;385:540-544
- Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an IκB kinase. Cell 1997;90:373-383
- Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DV. IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 1997;278:866-869
- Ling L, Cao Z, Goeddel D. NF-κB-inducing kinase activates IKK-α by phosphorylation of Ser-176. Proc Natl Acad Sci USA 1998;95:3792-3797
Datasheet 08-02W
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