|
Primary Antibodies
NGFR Antibody
Background
The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases (reviewed in 1). Nerve growth factor receptor (NGFR) was one of the earliest characterized members of this family (2). Also known as the low-affinity receptor p75NTR, this receptor is involved in several diverse functions such as apoptosis, neurite outgrowth during development, and myelination (reviewed in 3). Its ligands include NGF, brain-derived neurotrophic factor (BDNF), NT3, and NT4 (4). NGFR can also associate with other NGF receptors such as Trk through the cytosolic and transmembrane domains and thus can function as a co-receptor that refines Trk affinity and specificity for neurotrophins (5). Finally, upon binding of various neurotrophins, NGFR associates with tumor necrosis factor receptor-6 (TRAF6), suggesting that it can potentially function as a signal transducer for NGF signals through NGFR (6).
Additional Names
NGFR, p75NTR, TNFRSF16
|
Description
Left: Western blot analysis of (A) 25 ng, (B) 50 ng, and (C) 100 ng of purified recombinant NGFR with NGFR antibody at 1 μg/ml.
Below: Immunocytochemistry of NGFR in A20 cells with NGFR antibody at 10 μg/ml.
|
|
Other Product Images
|
Source
NGFR antibody was raised against purified recombinant human NGFR.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
NGFR antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Recombinant human NGFR
Application
NGFR antibody can be used for the detection of NGFR by Western blot at 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3593P - NGFR Peptide
Storage
NGFR antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1288 - A-20 Cell Lysate
Species Reactivity
H, M
Protein GI Number
4505393
Protein Accession Number
NP_002498
Short Description
low affinity nerve growth factor receptor
This product belongs to the following categories:
Related Products
References- Gaur U, Aggarwal BB. Regulation of proliferation, survival and apoptosis by members of the TNF superfamily. Biochem. Pharmacol. 2003; 66:1403-8.
- Johnson D, Lanahon A, Buck CR, et al. Expression and structure of the human NGF receptor. Cell 1986; 47:545-54.
- Gentry JJ, Barker, PA, and Carter BD. The p75 neuro-trophin receptor: multiple interactors and numerous functions. Prog. Brain Res. 2004;146:25-39.
- Nykjaer A, Willnow TE, and Petersen CM. p75NTR – live or let die. Curr. Opin. Neurobio. 2005; 15:49-57.
Datasheet 08-02W
|
|
