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Primary Antibodies
NANOG Antibody
Background
NANOG Antibody.
Additional Names
NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
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Description
Left: Western blot analysis of NANOG in HeLa (nuclear) cell lysate (35μg protein in RIPA buffer) using NANOG antibody (0.05μg/mL).
Below: NANOG staining of paraffin embedded human tonsil using NANOG antibody at 2μg/mL.
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Other Product Images
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Source
NANOG antibody was raised against a synthetic peptide near the C-terminus of NANOG.
Purification
NANOG antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
NANOG antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1μg/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources. Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2μg/ml.
Tested Application
E, WB, IHC
Buffer
NANOG antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-932P - NANOG Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. XBL-10433 - HeLa Nuclear Lysate
Species Reactivity
H
Protein Accession Number
NP_079141.1
Short Description
(CT) NANOG
This product belongs to the following categories:
Related Products
References- Yasuda SY, Tsuneyoshi N, Sumi T, Hasegawa K, Tada T, Nakatsuji N, Suemori H. NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer. Genes Cells. 2006 Sep;11(9):1115-23.
Datasheet 08-01W
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