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Primary Antibodies
NANOG Antibody
Background
NANOG Antibody.
Additional Names
NANOG, Nanog homeobox, HGNC:20857, homeobox transcription factor Nanog
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Description
Left: Western blot analysis of NANOG in human ovary lysate (35μg protein in RIPA buffer) using NANOG antibody (0.03μg/mL).
Below: NANOG staining (green) of a colony of induced pluriform stem cells derived from Human Keratinocytes using NANOG antibody (5μg/mL). Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.
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Other Product Images
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Source
NANOG antibody was raised against a synthetic peptide near an internal region of NANOG.
Purification
NANOG antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
NANOG antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.03-0.1μg/ml. Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Tested Application
E, WB, IF
Buffer
NANOG antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1316 - Human Ovary Tissue Lysate
Species Reactivity
H.M, R, D
Protein Accession Number
NP_079141.1
Short Description
(IN) NANOG
This product belongs to the following categories:
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References- Hyslop LA, Stojkovic M, Armstrong L, Walter T, Stojkovic P, Przyborski S, Herbert M, Murdoch A, Strachan T, Lako M. Downregulation of NANOG induces differentiation of Human Embryonic Stem Cells to Extraembryonic Lineages. Stem Cells. 2005 Jun 27; [Epub ahead of print]
Datasheet 08-01W
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