|
Primary Antibodies
NALP2 Antibody
Background
NALP2 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli (1). Unlike the prototypical NALP protein NALP1, NALP2 only contains a NACHT domain, leucine rich repeat (LRR), and pyrin-containing domain (PYD) (2). This protein interacts with the adapter protein ASC in addition to CARD8 and caspase-1 to form an inflammasome with high proIL-1Β-processing activity and is thought to function as a modulator of NF-ΚB and procaspase-1 activation in macrophages (2,3). It has also been suggested that NALP2, in addition to other NALP family members, can act as innate sensors of pathogens in a manner similar to the toll-like receptors (TLRs) (4). At least two alternatively spliced transcript variants encoding distinct isoforms have been found for NALP2.
Additional Names
NALP2, NACHT domain-, leucine-rich-repeat-, and PYD-containing protein 2, PYRIN-containing APAF1-like protein2, PAN1, PYPAF2
|
Description
Left: Western blot analysis of NALP2 in PC-3 cell lysate with NALP2 antibody at (A) 1 and (B) 2 μg/ml.
|
Source
NALP2 antibody was raised against a peptide corresponding to 12 amino acids near the carboxy-terminus of human NALP2.
Purification
Immunoaffinity chromatography purified IgG
Clonality / Clone
This is a polyclonal antibody.
Host
NALP2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human NALP2 Peptide (Cat. No. 3027P)
Application
NALP2 antibody can be used for detection of NALP2 by Western blot at 1 to 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3027P - NALP2 Peptide
Storage
NALP2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1216 - PC-3 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
8923473
Protein Accession Number
NP_060322
Short Description
An NF-κB modulating protein
This product belongs to the following categories:
Related Products
References- Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol. 2003; 4:95-104.
- Bruey JM, Bruey-Sedano N, Newman R, et al. PAN1/NALP2/PYPAF2, an inducible inflammatory mediator that regulates NF-κB and caspase-1 activation in macrophages. J. Biol. Chem. 2004; 279:51897-907.
- Agostini L, Martinon F, Burns K, et al. NALP3 forms an IL-1β-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 2004; 20:319-25.
- Martinon F and Tschopp J. NLRs join TLRs as innate sensors of pathogens. TRENDS Imm. 2005; 26:447-54.
Datasheet 08-02W
|
|
