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Primary Antibodies
NAK Antibody
Background
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. Phosphorylation of I-κB by IκB kinase (IKK) complex leads to degradation of I-κB and activation of NF-κB. The IKK complex contains IKKα, IKKβ, and IKKγ. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-κB-activating kinase), and T2K (1-3). NAK/TBK1 activates IKKβ through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-κB activation in response to growth factors (2). NAK/TBK1 functions upstream of NIK and the IKK complex (1,2). NAK/TBK1 is also critical in protecting embryonic liver from apoptosis (3).
Additional Names
NAK (CT), TBK1
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Description
Left: Immunocytochemistry of NAK in MOLT4 cells with NAK antibody at 10 μg/ml.
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Source
NAK antibody was raised against a synthetic peptide corresponding to 17 amino acids form near the carboxy terminus of human NAK/TBK1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
NAK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human NAK / TBK1 (C-Terminus) Peptide (Cat. No. 2351P)
Application
NAK antibody can be used for detection of NAK/TBK1 by Western blot at 0.5 to 1 μg/ml. An 84 kDa band should be detected. It is human, mouse and rat reactive and has no cross response to IKKa, IKKb, IKKy, or IKKe.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2351P - NAK Peptide
Storage
NAK antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - MOLT4 Cell Lysate
Species Reactivity
H, M
Protein GI Number
7019547
Protein Accession Number
NP_037386
Short Description
(CT) NF-kB activating kinase
This product belongs to the following categories:
Related Products
References- Pomerantz JL, Baltimore D. NF-κB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase. EMBO J 1999;18(23):6694-704
- Tojima Y, Fujimoto A, Delhase M, Chen Y, Hatakeyama S, Nakayama K, Kaneko Y, Nimura Y, Motoyama N, Ikeda K, Karin M, Nakanishi M. NAK is an IκB kinase-activating kinase. Nature 2000;404(6779):778-82
- Bonnard M, Mirtsos C, Suzuki S, Graham K, Huang J, Ng M, Itie A, Wakeham A, Shahinian A, Henzel WJ, Elia AJ, Shillinglaw W, Mak TW, Cao Z, Yeh WC. Deficiency of T2K leads to apoptotic liver degeneration and impaired NF-κB-dependent gene transcription. EMBO J. 2000;19(18):4976-85.
Datasheet 08-02W
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