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Primary Antibodies
NAIP Antibody
Background
Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy (1,2). Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR); these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules (reviewed in 3). Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO (4), suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported (5).
Additional Names
NAIP (CT), NAIP
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Description
Left: Western blot analysis of NAIP in PC-3 cell lysate with NAIP antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry of NAIP in A549 cells with NAIP antibody at 10 μg/ml.
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Other Product Images
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Source
NAIP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human NAIP.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
NAIP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human NAIP (C-Terminus) Peptide (Cat. No. 3315P)
Application
NAIP antibody can be used for the detection of NAIP by Western blot at 0.5 to 1 μg/ml. Minor, lower molecular weight bands may represent alternately spliced forms.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3315P - NAIP Peptide
Storage
NAIP antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1216 - PC-3 Cell Lysate
- Cat. No. 1203 - A549 Cell Lysate
Species Reactivity
H
Protein GI Number
1737213
Protein Accession Number
AAC52047
Short Description
(CT) Neuronal apoptosis inhibitor protein
This product belongs to the following categories:
Related Products
References- Roy N, Mahadevan MS, McLean M, et al. The gene for neuronal apoptosis inhibitory protein is partially deleted in individuals with spinal muscular atrophy. Cell 1995; 80:167-78.
- Liston P, Fong WG, Korneluk RG. The inhibitors of apoptosis: there is more to life than Bcl2. Oncogene 2003; 22:8568-80.
- Verhagen AM, Coulson EJ, and Vaux DL. Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs. Genome Biol. 2001; 2:reviews3009.1–reviews3009.10.
- Davoodi J, Lin L, Kelly J, et al. Neuronal apoptosis-inhibitory protein does not interact with Smac and requires ATP to bind caspase-9. J. Biol. Chem. 2004; 279:40622-8.
Datasheet 08-02W
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