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Primary Antibodies
Myeloperoxidase Antibody
Background
Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.
Additional Names
MPO, EC 1.11.1.7, myeloperoxidase, myeloperoxidase precursor
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Description
Left: Immunohistochemistry staining of Myeloperoxidase in spleen (formalin-fixed paraffin-embedded) using Myeloperoxidase antibody.
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Purification
Delipidation and Defibrination
Clonality / Clone
This is a polyclonal antibody.
Host
Myeloperoxidase antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Myeloperoxidase antibody was raised against Myeloperoxidase from human leukocytes. (Human)
Application
Myeloperoxidase antibody can be used for detection of Myeloperoxidase by WB, IP, ELISA, IF, IHC-parafin (1:500).
Tested Application
E, WB, IHC, IF, IP
Buffer
Myeloperoxidase antibody is supplied in PBS, pH 7.2, 0.01% sodium azide.
Storage
Myeloperoxidase antibody can be stored at 4˚C or -20˚C, Avoid repeated freezing and thawing.
Species Reactivity
H
Formulation
Volume: 50 μl
Concentration: 85 mg/ml
Short Description
Myeloperoxidase
This product belongs to the following categories:
Datasheet 09-01W
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