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17-701


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REDI-Ladder™ Molecular Weight Marker


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Mouse Cell Array

Background

Mouse cell lines (A-20, EL4, L1210, NIH/3T3, P815 and RAW 264.7)

Mouse Cell Array

Description

Mouse Cell Array

Preparation

Mouse Cell Array carries 6 different mouse cell lines, including A-20, EL4, L1210, NIH/3T3, P815, and RAW 264.7. Cells were harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4% paraformaldehyde. The cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well loaded with approximately 7.5 x 103 cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.

Application

Mouse Cell Array is provided for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunocytochemistry can be directly applied.

Tested Application

ICC

Storage

Mouse Cell Array can be stored for up to 3 months at 4°C under desiccate conditions.

Species Reactivity

n/a

Species

Mouse

Short Description

Mouse cell lines (A-20, EL4, L1210, NIH/3T3, P815 and RAW 264.7)

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