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Primary Antibodies
Metabotropic Glutamate Receptor 2/3 Antibody
Background
The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse. Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase.
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Description
Left: Metabotropic Glutamate Receptor 2/3 Antibody
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Purification
Affinity purified
Clonality / Clone
This is a polyclonal antibody.
Host
Metabotropic Glutamate Receptor antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Rabbit anti-mGluR 2/3 polyclonal antibody was raised against a peptide from the C-terminal region of mGluR2 and mGluR3. Rabbit anti-mGluR 2/3 by affinity purified on a column made with the peptide used as the antigen.
Application
Metabotropic Glutamate Receptor 2/3 antibody allows for strong and specific immunolabeling of ~125k mGluR5 and mGluR1a and the ~250k mGluR5 and mGluR1a dimers in Western blots of rat brain at antibody dilution of 1:1000. Immunolabeling blocked by preadsorption of antibody with the C-terminal peptide immunogen. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). Recognizes both mGluR5 and mGluR1a in rat brain. When internally tested under ideal conditions the working dilutions were 1:500 for IHC and 1:1000 for DB and WB.
Tested Application
WB, IHC
Buffer
Metabotropic Glutamate Receptor antibody is supplied as affinity purified polyclonal antibody, 200 μg antibody vial; lyophilized from ammonium bicarbonate (5 mM) so some residual salt may also be present, and there is an adequate amount of material to conduct 10-mini Western Blots.
Storage
Metabotropic Glutamate Receptor 2/3 antibody can be stored at -20˚C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20˚C. This product is stable at -20˚C for at least 1 year.
Species Reactivity
R
Short Description
Metabotropic Glutamate Receptor 2/3 Pan-Specific
This product belongs to the following categories:
References- Kim, S.J., Kim, Y,S, Yuan, J.P., Petralia, R.S., Worley, P.F. and Linden, D.J., “Activation of the TRPC1 cation channel by metabotropic glutamate receptor mGluR1”, Nature (London) 426 (2003) 285 - 291.
- Rammes, G., Palmer, M., Eder, M., Dodt, H.U., Zieglgansberger, W. and Collingridge, G.L., “Activation of mGlu receptors induces LTD without affecting postsynaptic sensitivity of CA1 neurons in rat hippocampal slices,” J. Physiol 546 (2003) 455 - 460.
- Ferré, S., Karcz-Kubicha, M., Hope, B.T., Popoli, P., Burgueño, J., Gutiérrez, M.S., Casadó, V., Fuxe, K., Goldberg, S.R., Lluis, C. Franco, R. and Ciruela, F., “Synergistic interaction between adenosine A2A and glutamate mGlu5 receptors: Implications for striatal neuronal function,” Proc. Natl. Acad. Sci. USA 99 (2002) 11,940 – 11,945.
- Huber, K.M., Kayser, M.S., and Bear, M.F., “Role for rapid dendritic protein synthesis in hippocampal mGluR-dependent long-term depression,” Science 288 (2000) 1254 - 1256.
Datasheet 0802-W
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