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Primary Antibodies
Mcl-1 Antibody
Background
Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival (reviewed in 1). While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak (2,3). Mcl-1 was originally identified in differentiating myeloid cells (4), but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis (5) and for the development and maintenance of B and T lymphocytes in animals (3). Mcl-1 exists as at least two distinct isoforms designated Mcl-1L and Mcl-1S. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death (6).
Additional Names
Mcl-1 (IN), Mcl-1
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Description
Left: Western blot analysis of Mcl-1 in Raji cell lysates with Mcl-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry of Mcl-1 in Raji cells with Mcl-1 antibody at 10 μg/ml.
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Other Product Images
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Source
Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the center of human Mcl-1.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
Mcl-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Virus Mcl-1 (Intermediate Domain) Peptide (Cat. No. 3481P)
Application
Mcl-1 antibody can be used for detection of both isoforms of Mcl-1 by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3481P - Mcl-1 Peptide
Storage
Mcl-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1207 - Raji Cell Lysate
Species Reactivity
H
Protein GI Number
11386165
Protein Accession Number
NP_068779
Short Description
(IN) a Bcl-2 protein family member
This product belongs to the following categories:
Related Products
References- Edwards SW, Derouet M, Howse M, et al. Regulation of neutrophil apoptosis by Mcl-1. Biochem Soc Trans. 2004; 32:489-92.
- Cuconati A, Mukherjee C, Perez D, et al. DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells. Genes and Dev. 2003; 17:2922-32.
- Opferman JT, Letai A, Beard C, et al. Development and maintenance of B and T lymphocytes require antiapoptotic MCL-1. Nature 2003; 426:671-6.
- Kozopas KM, Yang T, Buchan HL, et al. MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2. Proc. Natl. Acad. Sci. USA 1993; 90:3516-20.
Datasheet 08-02W
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