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Primary Antibodies
MYD88 Antibody
Background
The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway (1). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (2,3), which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
Additional Names
MYD88 (IN), MYD88
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Description
Left: Western blot analysis of MyD88 in Jurkat whole cell lysate with MyD88 antibody at 1 μg/ml.
Below: Immunohistochemical staining of human heart tissue using MyD88 antibody at 2 μg/ml.
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Other Product Images
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Source
MyD88 antibody was raised against a peptide corresponding to amino acids 233 to 248 of human MyD88, which differ from those of mouse by two amino acids.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
MYD88 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Human MyD88 (Intermediate Domain) Peptide (Cat. No. 2125P)
Application
MYD88 antibody can be used for detection of MyD88 by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate from Jurkat cells can be used as positive control and an approximately 35 kDa band can be detected. It is human and mouse reactive.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2125P - MYD88 Peptide
Storage
MYD88 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H, M
Protein GI Number
1763090
Protein Accession Number
U70451
Short Description
(IN) Adapter in IL-1 & LPS signal
This product belongs to the following categories:
AntibodiesPolyclonal AntibodiesSignal Transduction AntibodiesToll-like Receptor (TLR) Related AntibodiesInnate Immunity Antibodies
Related Products
References- Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997;278:1612-5
- Adachi O, Kawai T, Takeda K, Matsumoto M, Tsutsui H, Sakagami M, Nakanishi K, Akira S. Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function. Immunity 1998;9:143-50
Datasheet 08-02W
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