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Catalog Number:

45-095


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

MYD88 Antibody

Background

MYD88 Antibody.

Additional Names

MYD88, myeloid differentiation primary response gene (88)

MYD88 Antibody

Description

Left: Western blot analysis of MYD88 in human thymus lysate (RIPA buffer, 35μg total protein per lane) using MYD88 antibody (0.03μg/mL).

Below: Immunohistochemistry of MYD88 in paraffin embedded human tonsil using MYD88 antibody (3μg/mL).

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Source

MYD88 antibody was raised against a synthetic peptide at an internal region of MYD88.

Purification

MYD88 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

MYD88 antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 33kDa band observed in HumanThymus lysates (calculated MW of 33.2kDa according to NP_002459.1). Recommended concentration: 0.03-0.1μg/ml. Immunohistochemistry: In paraffin embedded tonsil, stains clusters of cells some of which are likely to be plasma cells. In the interfollicular area occasional strongly stained areas. Epithelium is weakly positive. Recommended concentration, 2-5μg/ml. Data obtained from previous batch.

Tested Application

E, WB, IHC

Buffer

MYD88 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Blocking Peptide

Cat. No. 45-095P - MYD88 Peptide

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Positive Control

  1. Cat. No. 1206 - MOLT4 Cell Lysate
  2. Cat. No. 1315 - Human Tonsil Tissue Lysate

Species Reactivity

H, R, M, D, Chm

Protein Accession Number

NP_002459.1

Short Description

(IN) MYD88

This product belongs to the following categories:

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References

  1. Li X, Qin J. Modulation of Toll-interleukin 1 receptor mediated signaling. J Mol Med. 2005 Jan 21; [Epub ahead of print]


Datasheet 08-02W

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