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XW-RP3155


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Recombinant Proteins

MTHFD2 Recombinant Protein

Background

This is a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD.

MTHFD2 Recombinant Protein

Description

SDS PAGE: Analysis of MTHFD2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.

Source

E. coli

Tested Application

E, WB, MS

Buffer

10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3

Storage

Store at -70˚C. As with any protein, exposing MTHFD2 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Species Reactivity

n/aH

Protein GI Number

5729935

Protein Accession Number

NP_006627

Domain Information

aa. 1-344

Molecular Weight

39.56 kDa (Calculated)

Fusion Partner

T7 tag at N-terminus

Purity

>95%

Short Description

Methylene tetrahydrofolate dehydrogenase 2 precursor, NAD-dependent

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Datasheet 0803-W

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