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Primary Antibodies
MELK Antibody
Background
MELK Antibody.
Additional Names
MELK, maternal embryonic leucine zipper kinase, KIAA0175, RP11-8N6.1, HPK38, OTTHUMP00000046113, pEg3 kinase
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Description
Left: Western blot analysis of MELK in human testis lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence using MELK antibody (0.1μg/mL).
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Source
MELK antibody was raised against a synthetic peptide near the C-terminus of MELK.
Purification
MELK antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
MELK antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: experiments gave bands at approx 75kDa and 150kDa in human testis lysates and in cell lines HeLa and Jurkat after 0.1µg/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3μg/ml.
Tested Application
E, WB
Buffer
MELK antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-089P - MELK Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1313 - Human Testis Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_055606.1
Short Description
(CT) MELK
This product belongs to the following categories:
Related Products
References- Nagase T, Seki N, Ishikawa K, Tanaka A, Nomura N. Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res 1996 Feb 29;3(1):17-24
Datasheet 08-02W
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