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Primary Antibodies
MEK1/2 (Phospho-Phospho-Ser218/222) Antibody
Background
MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK 1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade.
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Description
Left: MEK1/2 (Phospho-Phospho-Ser218/222) Antibody
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Purification
Affinity purified
Clonality / Clone
This is a polyclonal antibody.
Host
MEK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Rabbit anti-MEK 1/2 (Ser218/222) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer218/222 of human MEK 1/2. Rabbit anti- MEK 1/2 (Ser218/222) was purified by sequential chromatography on phospho-and non-phosphopeptide affinity columns.
Application
MEK1/2 antibody is specific for MEK 1/2 phosphorylated at Ser218/222. The antibody immunolabels the ~45k MEK protein in a Western blot of serum-stimulated NIH 3T3 cells. The immunolabeling is blocked by the phosphopeptide used as antigen but not the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Tested Application
WB
Buffer
MEK1/2 antibody is supplied as affinity purified polyclonal antibody, 100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol, and there is adequate amount of material to conduct 10-mini Western Blots.
Storage
For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Species Reactivity
H, M, R, X
Short Description
MEK1/2 (Ser218,222) Phospho-Specific
This product belongs to the following categories:
References- N.G. Ahn, N.G., Robbins, D.G., Haycock, J.W., Seger, R., Cobb, M.H. and Krebs, E.G., “Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin,” J. Neurochem. 59 (1992) 147 - 156.
- Crews, C.M., Alessandrini, A. and Erikson, R.L., “The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product,” Science 258 (1992) 478 - 480.
- Ahn, N.G. “The MAP kinase cascade. Discovery of a new signal transduction pathway,” Mol. Cell Biochem. 127 - 128 (1993) 201 - 209.
- Adams, J.P. and Sweatt, J.D., “Molecular psychology: Roles for the ERK MAP kinase cascade in memory,” Annu. Rev. Pharmacol. Toxicol. 42 (2002) 135 - 163.
Datasheet 0802-W
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