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Primary Antibodies
MED4 Antibody
Background
The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment. At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.
Additional Names
MED4, Mediator of RNA polymerase II transcription subunit 4, vitamin d receptor interacting protein, vdrip, drip36, trap36
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Description
Left: Western blot analysis of MED4 in human testis tissue lysate with MED4 antibody at 0.5 μg/ml.
Below: Immunohistochemistry of MED4 in human testis tissue with MED4 antibody at 10 μg/ml.
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Other Product Images
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Source
MED4 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human MED4.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
MED4 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
MED4 antibody can be used for detection of MED4 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4729P - MED4 Peptide
Storage
MED4 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1313 - Human Testis Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
7661788
Protein Accession Number
NP_054885
Short Description
Mediator of RNA polymerase II transcription subunit 4
This product belongs to the following categories:
Related Products
References- Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell 2004; 14:685-91.
- Gustafsson CM, Myers LC, Beve J, et al. Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:30851-4.
- Rachez C, Lemon BD, Suldan Z, et al. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature 1999; 398:824-8.
- Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.
Datasheet 08-04W
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