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Primary Antibodies
MECP2 Antibody
Background
Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Additional Names
MECP2, AUTSX3, DKFZp686A24160, MRX16, MRX79, PPMX, RTS, RTT
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Description
Left: Western blot analysis of MECP2 in Jurkat Cell Lysate using MECP2 antibody at 2.5 μg/ml (12% gel).
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Purification
Antibody is purified by protein A chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
MECP2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MECP2.
Application
MECP2 antibody can be used for detection of MECP2 by ELISA at 1:312500. MECP2 antibody can be used for detection of MECP2 by western blot at 2.5 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store MECP2 antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H, M, R, D
Protein Accession Number
NP_004983
Molecular Weight
52kDa
Short Description
MECP2 (methyl CpG binding protein 2 (Rett syndrome))
This product belongs to the following categories:
References- Robertson,L., (2006) Am. J. Med. Genet. B Neuropsychiatr. Genet. 141 (2), 177-183.
Datasheet 10-01W
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