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Primary Antibodies
MECP2 Antibody
Background
Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Additional Names
MECP2
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Description
Left: Western blot analysis of MECP2 expression in jurkat cell lysate using MECP2 antibody 2.5µg/ml.
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Purification
Rabbit IgG purified by protein A chromatography.
Clonality / Clone
This is a polyclonal antibody.
Host
MECP2 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human MECP2.
Application
MECP2 antibody can be used for Western blot at a suggested dilution at 2.5µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Tested Application
WB
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 100 ?l of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H, M, R, D
Protein Accession Number
NP_004983
Molecular Weight
52kDa
Short Description
Anti-MECP2
This product belongs to the following categories:
Related Products
References- Robertson,L., (2006) Am. J. Med. Genet. B Neuropsychiatr. Genet. 141 (2), 177-183
Datasheet 0802-W
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