|
Primary Antibodies
MECL1 Antibody
Background
MECL1 Antibody.
Additional Names
PSMB10, proteasome (prosome, macropain) subunit, beta type, 10, LMP10, MECL1, MGC1665, beta2i, macropain subunit MECl-1, multicatalytic endopeptidase complex subunit MECl-1, proteasome MECl-1, proteasome beta 10 subunit, proteasome catalytic subunit 2i, proteasome subunit MECL1, proteasome subunit beta 7i
|
Description
Left: Western blot analysis of MECL1 in human lung lysate (35μg protein in RIPA buffer) using MECL1 antibody (0.3μg/ml).
|
Source
MECL1 antibody was raised against a synthetic peptide cooresponding to the internal region of MECL1.
Purification
MECL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
MECL1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 26kDa band observed in Human, Liver, Lung and Lymph Node lysates (calculated MW of 28.9kDa according to NP_002792.1). Recommended concentration: 0.3-1µg/ml.
Tested Application
E, WB
Buffer
MECL1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 42-017P - MECL1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H
Protein Accession Number
NP_002792.1
Short Description
MECL1
This product belongs to the following categories:
References- Moschonas A, Kouraki M, Knox PG, Thymiakou E, Kardassis D, Eliopoulos AG. CD40 induces antigen transporter and immunoproteasome gene expression in carcinomas via the coordinated action of NF-kappaB and of NF-kappaB-mediated de novo synthesis of IRF-1. Molecular and Cellular Biology 2008 Oct 28 (20): 6208-22.
Datasheet 10-01W
|
|
