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Primary Antibodies
MDA5 Antibody
Background
The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
Additional Names
MDA5 (CT), Melanoma differentiation-associated protein 5, Interferon-induced with helicase C domain protein 1, IFIH1
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Description
Left: Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 μg/ml.
Below: Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 μg/ml.
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Other Product Images
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Source
MDA5 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human MDA5.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
MDA5 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
MDA5 antibody can be used for detection of MDA5 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4037P - MDA5 Peptide
Storage
MDA5 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
- Cat. No. 1369 - Human Lymph Node Tissue Lysate
Species Reactivity
H, M
Protein GI Number
27886568
Protein Accession Number
NP_071451
Short Description
(CT) Melanoma differentiation-associated protein 5
This product belongs to the following categories:
Related Products
References- Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.
- Hiscott J, Nguyen T-LA, Arguello M, et al. Manipulation of the nuclear factor-?B pathway and the innate immune response by viruses. Oncogene 2006; 25:6844-67.
- Kang D, Gopalrishnan RV, Lin L, et al. Expression analysis and genomic characterization of human melanoma differentiation associated gene-5, mda-5: a novel type I interferon-responsive apoptosis-inducing gene. Oncogene 2004; 23:1789-800.
- Andrejeva J, Childs KS, Young DF, et al. The V proteins of the paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter. Proc. Natl. Acad. Sci. USA 2004; 101:17264-9.
Datasheet 08-02W
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