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Primary Antibodies
MDA5 Antibody
Background
MDA5 Antibody.
Additional Names
IFIH1, MDA5, MDA-5, Hlcd, MGC133047, IDDM19, melanoma differentiation associated protein-5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, interferon induced with helicase C domain 1
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Description
Left: Western blot analysis of MDA5 in paraffin embedded human pancreas using MDA5 antibody (10μg/mL).
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Source
MDA5 antibody was raised against a synthetic peptide of MDA5.
Purification
MDA5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
MDA5 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution >1:32,000. Western Blot: No signal obtained yet but low background observed in human placenta lysate at up to 1μg/ml.
Tested Application
E, IHC
Buffer
MDA5 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-874P - MDA5 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1307 - Human Pancreas Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_071451.2
Short Description
MDA5 / IFIH1
This product belongs to the following categories:
Related Products
References- Kang DC, Gopalkrishnan RV, Wu Q, Jankowsky E, Pyle AM, Fisher PB. mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties. Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):637-42.
Datasheet 08-01W
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