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Slides
MCF7 Cell Slide
Background
MCF7 (breast; epithelial; pleural effusion adenocarcinoma)
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Description
MCF7 Cell Slide
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Source
human breast epithelial adenocarcinoma
Preparation
Human MCF7 cells were cultured in Ham's F12K medium and harvested at the log phase of growth. The MCF7 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 MCF7 cells. A follow-up heat dehydration process was done to ensure the attachment of MCF7 cells and the stability of cellular proteins.
Application
MCF7 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested. Optimal conditions should be determined by the end user.
Tested Application
ICC
Storage
MCF7 cell slide can be stored for up to 6 months at 4°C under desiccate conditions.
Species Reactivity
n/a
Species
Human
Short Description
Human mammary gland, breast, epithelial adenocarcinoma
This product belongs to the following categories:
Datasheet 0803-W
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