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Primary Antibodies
MAPKAP1 Antibody
Background
MAPKAP1 was initially identified as the human homolog of S. pombe SIN1. Recent evidence has shown that it identical to Mip1, a protein that interacts with MEKK2, a member of the mitogen-activated protein kinase (MAPK) intracellular signaling network. MAPKAP1 is thought to prevent MEKK2 activation and dimerization by forming a complex with the inactive and non-phosphorylated MEKK2, thereby blocking the JNK1/2, ERK1/2, p38 and ERK5 MAPKs. MAPKAP1 has also been shown to play a role in the TOR signaling process, a pathway that is involved in controlling cell growth and proliferation in response to environmental cues such as nutrients, growth factors and hormones. Experiments showed that MAPKAP1 helped to maintain the TOR/rictor assembly but not the TOR/RAPTOR complex, which allowed specific phosphorylation of Akt, a kinase that is believed to couple the growth factor-PI3K signaling pathway to the nutrient-regulated TOR signaling pathway. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.
Additional Names
MAPKAP1 (IN), Mitogen activated protein kinase associated protein 1, Sin1, stress-activated protein kinase-interacting protein 1, MIP1
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Description
Left: Western blot analysis of MAPKAP1 in human skeletal muscle tissue lysate with MAPKAP1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of MAPKAP1 in human skeletal muscle tissue with MAPKAP1 antibody at 5 μg/ml.
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Other Product Images
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Source
MAPKAP1 antibody was raised against an 18 amino acid peptide from near the center of human MAPKAP1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
MAPKAP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
MAPKAP1 antibody can be used for detection of MAPKAP1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4079P - MAPKAP1 Peptide
Storage
MAPKAP1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
56788407
Protein Accession Number
NP_001006618
Short Description
(IN) Mitogen activated protein kinase associated protein 1
This product belongs to the following categories:
Related Products
References- Schroder W, Cloonan N, Bushell G, et al. Alternative polyadenylation and splicing of mRNAs transcribed from the human Sin1 gene. Gene 2004; 339:17-23.
- Cheng J, Zhang D, Kim K, et al. Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and activation. Mol. Cell. Biol. 2005; 25:5955-64.
- Jacinto E, Facchinetti V, Liu D, et al. SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 2006; 127:125-37.
- Wullschleger S, Loewith R and Hall MN. TOR signaling in growth and metabolism. Cell 2006; 124:471-84.
Datasheet 08-02W
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