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Primary Antibodies
MANF Antibody
Background
MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson’s disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress. This antibody does not cross-react with CDNF.
Additional Names
MANF (NT), Mesencephalic astrocyte-derived neurotrophic factor, arginine-rich mutatedin early stage tumor, ARMET, ARP
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Description
Left: Western blot analysis of MANF in rat brain tissue lysate with MANF antibody at (A) 1 and (B) 2 μg/ml.
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Source
MANF antibody was raised against a 12 amino acid peptide from near the amino terminus of human MANF.
Clonality / Clone
This is a polyclonal antibody.
Host
MANF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
MANF antibody can be used for detection of MANF by Western blot at 1 – 2 μg/ml.
Tested Application
WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
MANF antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H,M,R
Protein Accession Number
P55145
Data Sheet Version
08-02W
Related Products
References
- Shridhar V, Rivard S, Shridhar R, et al. A gene from human chromosomal band 3p21.1 encodes a highly conserved arginine-rich protein and is mutated in renal cell carcinomas. Oncogene 1996; 12:1931-9.
- Shridhar R, Shridhar V, Rivard S, et al. Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck. Cancer Res. 1996; 56:5576-8.
- Petrova P, Raibekas A, Pevsner J, et al. MANF: a new mesencephalic, astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons. J. Mol. Neurosci. 2003; 20:173-88.
- Lindholm P, Voutilainen MH, Lauren J, et al. Novel neurotrophic factor CDNF protects and rescues midbrain dopamine neurons in vivo. Nature 2007; 448:73-7.
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