|
Primary Antibodies
MAK10 Antibody
Background
The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.
Additional Names
MAK10 (NT), amino-acid N-acetyltransferase subunit, Embryonic growth-associated protein homolog, EGAP
|
Description
Left: Western blot analysis of MAK10 in rat heart tissue lysate with MAK10 antibody at 1 μg/ml in the (A) absence and (B) presence of blocking peptide.
Below: Immunohistochemistry of MAK10 in human heart tissue with MAK10 antibody at 2.5 μg/ml.
|
|
Other Product Images
|
Source
MAK10 antibody was raised against a 19 amino acid peptide near the amino terminus of human MAK10.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
MAK10 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
MAK10 antibody can be used for detection of MAK10 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5271P - MAK10 Peptide
Storage
MAK10 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1461 - Rat Heart Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
145275204
Protein Accession Number
NP_078911
Short Description
(NT) Embryonic growth-associated protein homolog
This product belongs to the following categories:
References- Lee YJ et al. MAK10, a glucose repressible gene necessary for replication of a dsRNA virus of Saccharomyces cerevisiae, has T cell receptor alpha-subunit motifs. Genetics 1992; 132:87-96.
- Yi XJ, et al. A novel epithelial wound-related gene is abundantly expressed in developing rat cornea and skin. Curr. Eye Res. 2000; 20:430-40.
- Wenzlau JM, et al. Embryonic growth-associated protein is one subunit of a novel N-terminal acetyltransferase complex essential for embryonic vascular development. Circ. Res. 2006; 98:846-55.
- Weiser-Evans MC, et al. Novel embryonic genes are preferentially expressed by autonomously replicating rat embryonic and neointimal smooth muscle cells. Circ. Res. 2000; 87:608-15.
Datasheet 09-01W
|
|
