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Primary Antibodies
MADH6 Antibody
Background
Antagonist of signaling by TGF-β (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1.SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
Additional Names
MADH6, MADH7
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Description
Left: Western blot analysis of SMAD6 in Jurkat cell lysate using anti-SMAD6.
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Purification
Rabbit total IgG
Clonality / Clone
This is a polyclonal antibody.
Host
MADH6 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
MADH6 antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human MADH6.
Application
MADH6 antibody can be used for Western blot at a suggested dilution of 0.5-1.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,052 as secondary antibody. The protein size is 496 amino acids.
Tested Application
WB, IHC
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
MADH6 antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_005576
Molecular Weight
53 kDa
Short Description
Anti-SMAD6
This product belongs to the following categories:
Related Products
References- Tsunobuchi,H., et al., (2004) Biophys. Res. Commun. 316(3),712-719
Datasheet 0802-W
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