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Primary Antibodies
MADD Antibody
Background
MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94% overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87% identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.
Additional Names
MADD (CT), DENN
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Description
Left: Western blot analysis of MADD in whole cell lysates from the indicated cell lines with MADD antibody at 1:250 dilution.
Below: Immunocytochemistry of MADD in human spleen tissue with MADD antibody at 10 μg/ml.
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Other Product Images
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Source
MADD antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human MADD.
Purification
Antibody is DEAE purified
Clonality / Clone
This is a polyclonal antibody.
Host
MADD antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Bacteria MADD (C-Terminus) Peptide (Cat. No. 1150P)
Application
MADD antibody can be used for detection of MADD by Western blot at 1:250 to 1:500 dilution. 200 to 220 kDa bands should be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1150P - MADD Peptide
Storage
MADD antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
- Cat. No. 1212 - 3T3 Whole Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
3289973
Protein Accession Number
AAD12154
Short Description
(CT) Activator of MAPK pathway
This product belongs to the following categories:
Related Products
References- Schievella AR, Chen JH, Graham JR, Lin LL. MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase. J Biol Chem 1997;272:12069-12075
- Chow VT, Lee SS. DENN, a novel human gene differentially expressed in normal and neoplastic cells. DNA Seq 1996;6:263-273
- Zhang Y, Zhou L, Miller CA. A splicing variant of a death domain protein that is regulated by a mitogen-activated kinase is a substrate for c-Jun N-terminal kinase in the human central nervous system. Proc Natl Acad Sci U S A 1998;95:2586-2591
- Brown TL and Howe PH. MADD is highly homologous to a Rab3 guanine-nucleotide exchange protein (Rab3-GEP). Curr Biol 1998;8:R191
Datasheet 08-02W
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