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Primary Antibodies
Liver X Receptor Antibody
Background
Liver X Receptor Antibody. This antibody is expected to recognize an epitope corresponding to aa 429-442 of human LXR alpha protein (NP_005684) and to aa 443-456 of human LXR beta protein (NP_009052).
Additional Names
NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N
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Description
Left: Western blot analysis of liver X Receptor in human spleen lysate (35μg protein in RIPA buffer) using liver X Receptor antibody (1μg/mL).
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Source
Liver X Receptor antibody was raised against a synthetic peptide at an internal region of Liver X Receptor.
Purification
Liver X Receptor antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Liver X Receptor antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approx 55kDa band observed in human spleen lysates (calculated MW of 50.5kDa according to NP_005684 and 50.1kDa according to NP_009052). Recommended concentration: 1-3μg/ml.
Tested Application
E, WB
Buffer
Liver X Receptor antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-100P - Liver X Receptor Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein Accession Number
NP_005684, NP_009052
Short Description
(IN) Liver X Receptor / LXR alpha / LXR beta
This product belongs to the following categories:
Related Products
References- Tangirala RK, Bischoff ED, Joseph SB, Wagner BL, Walczak R, Laffitte BA, Daige CL, Thomas D, Heyman RA, Mangelsdorf DJ, Wang X, Lusis AJ, Tontonoz P, Schulman IG. Identification of macrophage liver X receptors as inhibitors of atherosclerosis. Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11896-901. Epub 2002 Aug 22.
Datasheet 08-02W
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