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Primary Antibodies
Lck Monoclonal Antibody
Background
Lck(lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.
Additional Names
YT16, p56lck, pp58lck
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Description
Western blot analysis using LCK monoclonal antibody against truncated LCK recombinant protein and Jurkat cell lysate.
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Clonality / Clone
This is a monoclonal antibody. Clone 8E5F9, 4B2C7.
Isotype
IgG2a
Host
Lck monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified truncated recombinant Lck expressed in E. Coli strain BL21 (DE3).
Application
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
E, WB
Storage
Lck monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
3932
Short Description
lymphocyte-specific protein tyrosine kinase
This product belongs to the following categories:
Related Products
References- Mingjian Shi, John C. Cooper, Chao-Lan Yu. Mol. Cancer Res., Jan 2006; 4: 39–45
- Robert F. Stachlewitz, Michelle A. Hart, Brian Bettencourt. J. Pharmacol. Exp. Ther., Oct 2005; 315: 36–41.
- Ibrahim Y. Hawash, Kamala P. Kesavan, Anthony I. Magee. J. Biol. Chem., Feb 2002; 277: 5683–5691.
Datasheet 0903-W
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