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Primary Antibodies
LSD1 Antibody
Background
Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction (1). Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription (2). LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation (3,4). Experiments have shown that CoREST, a SANT domain-containing corepressor (5) acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein (6), inhibits CoREST/LSD1 activity in vitro (3). LSD1-mediated histone demethylation thus may have significant effects on gene expression.
Additional Names
LSD1 (IN), Lysine-specific histone demethylase, amine oxidase flavin containing domain protein 2, AOF2
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Description
Left: Western blot analysis of LSD1 in P815 cell lysate with LSD1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of LSD1 in human small intestine tissue with LSD1 antibody at 2 μg/ml.
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Other Product Images
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Source
LSD1 antibody was raised against a 16 amino acid peptide from near the center of human LSD1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
LSD1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human LSD1 (Intermediate Domain) Peptide (Cat. No. 3763P)
Application
LSD1 antibody can be used for the detection of LSD1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3763P - LSD1 Peptide
Storage
LSD1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1286 - P815 Cell Lysate
- Cat. No. 1308 - Human Small Intestine Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
58761544
Protein Accession Number
NP_001009999
Short Description
(IN) A histone demethylase
This product belongs to the following categories:
Related Products
References- Shi Y, Lan F, Matson C, et al. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 2004; 119:941-53.
- Kouzarides T. Histone methylation in transcriptional control. Curr. Opin. Genet. Dev. 2002; 12:198-209.
- Shi YJ, Matson C, Lan F, et al. Regulation of LSD1 histone demethylase activity by its associated factors. Mol. Cell 2005; 19:857-64.
- Nakamura T, Mori T, Tada S, et al. ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation. Mol. Cell 2002; 10:1119-28.
Datasheet 08-02W
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