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Primary Antibodies
LGP2 Antibody
Background
Anti-viral innate immune responses are triggered by pathogen-associated molecular patters (such as the accumulation of intracellular nucleic acids resulting from virus infections) and represent the first line of defense against numerous infectious organisms. The Toll-like receptors (TLR) 3, 7, 8, and 9 are expressed in immune cells and function as transmembrane pattern recognition receptors to detect foreign nucleic acids. Other proteins that play similar roles, such as RIG-1 and MDA5 are members of a CARD-helicase family and are expressed in the cytoplasm. A third protein, LGP2, is similar to RIG-1 and MDA5, except for lacking a homologous CARD domain. It is thought to act as an element of negative-feedback regulation of intracellular antiviral signaling. At least four isoforms of LGP2 are known to exist.
Additional Names
LGP2 (NT), DHX58, likely ortholog of mouse D11lgp2
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Description
Left: Western blot analysis of LGP2 in MDA-MB-361 cell lysate with LGP2 antibody at (A) 0.5 and (B) 1 μg/ml.
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Source
LGP2 antibody was raised against a 14 amino acid peptide from near the amino terminus of human LGP2.
Clonality / Clone
This is a polyclonal antibody.
Host
LGP2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
LGP2 antibody can be used for detection of LGP2 by Western blot at 0.5 – 1 μg/ml.
Tested Application
WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
LGP2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1217 - MDA-MB-361 Cell Lysate
Species Reactivity
H,M,R
Protein Accession Number
EAW60798
Data Sheet Version
08-02W
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References
- Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.
- Iwasaki A and Medzhitov R. Toll-like receptor control of the adaptive immune responses. Nat. Immunol. 2004; 5:987-95.
- Yoneyama M, Kikuchi K, Matsumoto K, et al. The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol. 2004; 5:730-7.
- Andrejeva J, Childs KS, Young DF, et al. The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibits its activation of the IFN-betta promoter. Proc. Natl. Acad. Sci. USA, 2004; 101:17264-9.
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