|
Primary Antibodies
LGI1 Antibody
Background
The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist, but the top band seen in the western blot is likely to be non-specific. This LGI1 antibody is predicted to be specific to LGI1 and not recognize other LGI proteins.
Additional Names
LGI1 (NT), Leucine-rich, glioma inactivated 1, epitempin, EPT
|
Description
Left: Western blot analysis of LGI1 in mouse brain tissue lysate with LGI1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
|
Source
LGI1 antibody was raised against a 13 amino acid peptide from near the amino terminus of human LGI1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
LGI1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
LGI1 antibody can be used for the detection of LGI1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4531P - LGI1 Peptide
Storage
LGI1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1403 - Mouse Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
37182888
Protein Accession Number
AAQ89244
Short Description
(NT) Leucine-rich, glioma inactivated 1
This product belongs to the following categories:
Related Products
References- Chernova OB, Somerville RP and Cowell JK. A novel gene, LGI1, from 10q24 is rearranged and downregulated in malignant brain tumors. Oncogene 1998; 17:2873-81.
- Fialka F, Gruber RM, Hitt R, et al. CPA6, FMO2, LGI1, SIAT1 and TNC are differentially expressed in early- and late-stage oral squamous cell carcinoma – A pilot study. Oral Oncol. 2008;
- Gu W, Gibert Y, Wirth T, et al. Using gene-history and expression analysis to assess the involvement of LGI genes in human disorders. Mol. Biol. Evol. 2005; 22:2209-16.
- Fukata Y, Adesnik H, Iwanaga T, et al. Epilepsy-related ligand/receptor complex LGI1 and ADAM22 regulate synaptic transmission. Science 2006; 313:1792-5.
Datasheet 08-02W
|
|
