|
Primary Antibodies
LEFTY2 Monoclonal Antibody
Background
During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
Additional Names
LEFTY2
|
Description
Left: Immunohistochemistry staining of LEFTY2 in uterus, myometrium (formalin-fixed paraffin-embedded) using LEFTY2 monoclonal antibody.
Below: LEFTY2 monoclonal antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. 1.0 mg of protein of protein was loaded per lane. The blot was incubated with a 1:2,000 dilution of LEFTY2 monoclonal antibody.
|
|
Other Product Images
|
Purification
Protein A Column
Clonality / Clone
This is a monoclonal antibody.
Host
LEFTY2 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
LEFTY2 monoclonal antibody was raised against recombinant LEFTY2. (Human)
Application
LEFTY2 monoclonal antibody can be used for detection of LEFTY2 by WB, ELISA, IHC-parafin (10 μg/ml).
Tested Application
E, WB, IHC
Buffer
LEFTY2 monoclonal antibody is supplied in 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
LEFTY2 monoclonal antibody can be stored at 4˚C or -20˚C. Avoid repeated freezing and thawing.
Species Reactivity
M, R
Formulation
Weight: 50 μg
Short Description
Endometrial Bleeding Associated Factor
This product belongs to the following categories:
Datasheet 09-01W
|
|
