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Catalog Number:

45-082


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

LDLRAP1 Antibody

Background

LDLRAP1 Antibody.

Additional Names

ARH, ARH2, FHCB1, FHCB2, MGC34705, DKFZp586D0624, LDL receptor adaptor protein, low density lipoprotein receptor adaptor protein 1

LDLRAP1 Antibody

Description

Left: Western blot analysis of LDLRAP1 in human liver lysate (RIPA buffer, 30μg total protein per lane) using LDLRAP1 antibody (3μg/mL).

Source

LDLRAP1 antibody was raised against a synthetic peptide near the N-terminus of LDLRAP1.

Purification

LDLRAP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

LDLRAP1 antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approx 40-45kDa band observed in human liver lysate (predicted MW of 35kDa according to NP_056442.1). Recommended for use at 1-3μg/ml.

Tested Application

E, WB

Buffer

LDLRAP1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Blocking Peptide

Cat. No. 45-082P - LDLRAP1 Peptide

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Positive Control

  1. Cat. No. 1304 - Human Liver Tissue Lysate

Species Reactivity

H, M

Protein Accession Number

NP_056442.1

Short Description

(NT) ARH / LDL receptor adaptor

This product belongs to the following categories:

Related Products

References

  1. He G, Gupta S, Yi M, Michaely P, Hobbs HH, Cohen JC. ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2. J Biol Chem. 2002 Nov 15;277(46):44044-9.


Datasheet 08-02W

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