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Primary Antibodies
LDL-R Antibody
Background
The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in the LDL-R gene cause the autosomal dominant disorder, familial hypercholesterolemia. Along with SCARB1, CLDN1, and the tetraspanin superfamily member CD81, LDL-R has been reported to be an entry factor for the Hepatitis C virus. At least three isoforms of LDL-R are known to exist.
Additional Names
LDL-R, Low density lipoprotein receptor, LDLR, FH, FHC, LDLCQ2
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Description
Left: Western blot analysis of LDL-R in human liver tissue lysate with LDL-R antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of LDL-R in human liver tissue with LDL-R antibody at 2.5 μg/ml.
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Other Product Images
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Source
LDL-R antibody was raised against an 18 amino acid peptide near the center of human LDL-R.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
LDL-R antibody was raised in chicken.
Please use anti-chicken secondary antibodies.
Application
LDL-R antibody can be used for detection of LDL-R by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5163P - LDL-R Peptide
Storage
LDL-R antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
4504975
Protein Accession Number
NP_000518
Short Description
Low density lipoprotein receptor
This product belongs to the following categories:
References- Li Y, Cam J, and Bu G. Low-density lipoprotein receptor family: endocytosis and signal transduction. Mol. Neurobiol. 2001; 23:53-67.
- Austin MA, Hutter CM, Zimmern RL, et al. Familial hypercholesterolemia and coronary heart disease: a HuGE association review. Am. J. Epidemiol. 2004; 160:421-9.
- Helle F and Dubuisson J. Hepatitis C virus entry into host cells. Cell Mol. Life Sci. 2008; 65:100-12.
Datasheet 09-01W
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