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Primary Antibodies
LAMP-1 Antibody
Background
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome (4,5). LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression (6). However, the loss of both proteins resulted in embryonic lethality (7), suggesting that each protein possesses some unique and necessary functions.
Additional Names
LAMP-1, Lysosome associated membrane protein 1
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Description
Left: Western blot analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunofluorescence of LAMP-1 in human colon tissue with LAMP-1 antibody at 20 μg/ml.
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Other Product Images
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Source
LAMP-1 antibody was raised against a 15 amino acid peptide from near the center of human LAMP-1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
LAMP-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human LAMP-1 Peptide (Cat. No. 3629P)
Application
LAMP-1 antibody can be used for the detection of LAMP-1 by Western blot at 1 – 2 μg/ml. (Optimal dilution should be determined by user). Despite its predicted size, LAMP-1 migrates at 110kDa in SDS-PAGE.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3629P - LAMP-1 Peptide
Storage
LAMP-1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1287 - EL4 Cell Lysate
- Cat. No. 1320 - Human Colon Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
112380628
Protein Accession Number
NP_005552
Short Description
An autophagy related protein
This product belongs to the following categories:
Related Products
References- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
- Chen JW, Murphy TL, Willingham MC, et al. Identification of two lysosomal membrane glycoproteins. J. Cell Biol. 1985; 101:85-95.
Datasheet 08-02W
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