|
Primary Antibodies
KappaB ras2 Antibody
Background
KappaB ras-1 (κB-ras-1) and κB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IkappaB (IκB), an inhibitor of the transcription factor NF-κB. IκB exists in two homologous forms, IκB-α and IκB-β, although IκB-β contains a unique 47-amino acid region within its ankyrin domain. While inactive IκB-α-NF-κB complexes can shuttle in and out of the nucleus, IκB-β-NF-κB complexes are retained exclusively in the cytoplasm. It is suggested that κB-ras proteins preferentially bind to the IκB-β form through this unique insert within the ankyrin region, thus modulating the cellular location of IκB-β and regulating the rate of degradation of IκB-β. This antibody is specific for κB-ras2 and has no cross-reactivity to κB-ras1.
Additional Names
KappaB ras2, NF-kappaB inhibitor-interacting Ras like protein 2, kappaB-ras2, NKIRAS2
|
Description
Left: Western blot analysis of KappaB ras2 in RAW264.7 cell lysate with KappaB ras1 antibody at 1 μg/ml in the (A) absence and (B) presence of blocking peptide.
|
Source
KappaB ras2 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human KappaB ras2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
KappaB ras2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
KappaB ras2 antibody can be used for detection of KappaB ras1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 2495P - KappaB ras2 Peptide
Storage
KappaB ras2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1283 - RAW264.7 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
19072794
Protein Accession Number
NP_060065
Short Description
NF-κB inhibitor-interacting Ras-like protein
This product belongs to the following categories:
Related Products
References- Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73.
- Chen Y, Wu J and Ghosh G. KappaB-Ras binds to the unique insert within the ankyrin repeat domain if IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta x NF-kappaB complexes. J. Biol. Chem. 2003; 278:23101-6.
Datasheet 08-02W
|
|
