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Slides
Jurkat Cell Slide
Background
Jurkat, Clone E6-1 (T lymphocyte; acute T cell leukemia)
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Description
Jurkat Cell Slide
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Source
human acute T cell leukemia
Preparation
Human Jurkat, Clone E6-1 cells were cultured in RPMI 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4% paraformaldehyde. The Jurkat cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 Jurkat cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins.
Application
Jurkat cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested. Optimal conditions should be determined by the end user.
Tested Application
ICC
Storage
Jurkat cell slide can be stored for up to 6 months at 4°C under desiccate conditions.
Species Reactivity
n/a
Species
Human
Short Description
Human T lymphocyte acute T cell leukemia
This product belongs to the following categories:
Datasheet 0803-W
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