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Primary Antibodies
JMJD1C Antibody
Background
The jumonji domain containing 1C protein (JMJD1C) was initially discovered in silico, and later suggested to be a candidate gene for autism. Like the related proteins JMJD1A and JMJD1B, JMJD1C is a histone H3K9 demethylase implicated in the nuclear hormone receptor-based transcriptional regulation. JMJD1C mRNA is highly expressed in undifferentiated embryonic stem (ES) cells as well as pancreatic islet, diffuse-type gastric cancer, and other tissues and tumors. The JMJD1C gene promoter contain bHLH-, AP-1-, and POU5F1-binding sites, and as preferential expression of POU5F1 has been reported in ES cells, pancreatic islet, and diffuse-type gastric cancer, it has been suggested that POU5F1-mediated expression of JMJD1C reactivates previously silenced genes in ES cells and diffuse-type gastric cancer. At least three isoforms of JMJD1C are known to exist. This antibody will not cross-react with JMJD1A or JMJD1B.
Additional Names
JMJD1C,Jumonji domain containing 1C protein, TRIP8
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Description
Left: Western blot analysis of JMJD1C in human liver tissue lysate with JMJD1C antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of JMJD1C in rat liver tissue with JMJD1C antibody at 2.5 μg/ml.
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Other Product Images
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Source
JMJD1C antibody was raised against a 20 amino acid peptide from near the amino terminus of human JMJD1C.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
JMJD1C antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
JMJD1C antibody can be used for detection of JMJD1C by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5371P - JMJD1C Peptide
Storage
JMJD1C antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
118600981
Protein Accession Number
NP_116165
Short Description
Jumonji domain containing 1C protein
This product belongs to the following categories:
References- Katoh M and Katoh M. Identification of TRIP8 gene in silico. Int. J. Mol. Med. 2003; 12:817-21.
- Castermans D, Vermeesch JR, Fryns JP, et al. Identification and characterization of the TRIP8 and REEP3 genes on chromosome 10q21.3 as novel candidate genes for autism. Eur. J. Hum. Genet. 2007; 15:422-31.
- Katoh M and Katoh M. Comparative integromics on JMJD1C gene encoding histone demethylase: Conserved POU5F1 binding site elucidating mechanism of JMJD1C expression in undifferentiated ES cells and diffuse-type gastric cancer. Int. J. Oncology 2007; 31:219-23.
- Katoh Y and Katoh M. Conserved POU-binding site linked to SP1-binding site within FZD5 promoter: transcriptional mechanism of FZD5 in undifferentiated human ES cells, fetal liver/spleen, adult colon, pancreatic islet, and diffuse-type gastric cancer. Int. J. Oncol. 2007; 30:751-5.
Datasheet 09-01W
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