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Primary Antibodies
JMJD1B Antibody
Background
The jumonji domain containing 1B protein (JMJD1B) was originally discovered as a candidate for the myeloid leukemia tumor suppressor gene. Besides CD34+ cells and acute myeloid leukemia (AML) cell lines, JMJD1B mRNA is highly expressed in liver, heart, kidney, skeletal muscle, and placenta tissues. The JMJD1B gene is localized to a region of chromosome 5q31, which is frequently deleted in myeloid leukemias and myelodysplasias and expression of JMJD1B in a del(5q) cell line results in suppression of clonogenic growth suggesting that JMJD1B may function as a tumor suppressor. In contrast, JMJD1B gene copy number and mRNA expression level was increased in several non-small cell lung cancers indicating that the role of JMJD1B in cancer formation and progression is more complex than originally postulated. At least three isoforms of JMJD1B are known to exist. This antibody will not cross-react with JMJD1A or JMJD1C.
Additional Names
JMJD1B,5qNCA, Lysine-specific demethylase 3B, KDM3B
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Description
Left: Western blot analysis of JMJD1B in rat liver tissue lysate with JMJD1B antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of JMJD1B in rat liver tissue with JMJD1B antibody at 2.5 μg/ml.
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Other Product Images
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Source
JMJD1B antibody was raised against a 20 amino acid peptide from near the center of human JMJD1B.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
JMJD1B antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
JMJD1B antibody can be used for detection of JMJD1B by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5369P - JMJD1B Peptide
Storage
JMJD1B antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1464 - Rat Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
119582545
Protein Accession Number
EAW62141
Short Description
Lysine-specific demethylase 3B
This product belongs to the following categories:
References- Lai F, Godley LA, Fernald AA, et al. cDNA cloning and genomic structure of three genes localized to human chromosome band 5q31 encoding potential nuclear proteins. Genomics 2000; 70:123-30.
- Hu Z, Gomes I, Horrigan SK, et al. A novel nuclear protein, 5qNCA (LOC51780) is a candidate for the myeloid leukemia tumor suppressor gene on chromosome band q31. Oncogene 2001; 20:6946-54.
- Baik S-H, Jee B-K, Choi J-S, et al. DNA profiling by array comparative genomic hybridization (CGH) of peripheral blood mononuclear cells (PBMC) and tumor tissue cell in non-small cell lung cancer (NSCLC). Mol. Biol. Rep. 2009; 36:1767-78.
Datasheet 09-01W
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