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Primary Antibodies
JMJD1A Antibody
Background
The jumonji domain containing 1A protein (JMJD1A) was originally discovered as a testes specific gene, but has been found to be expressed in numerous tissues. JMJD1A is a histone demethylase and specifically demethylates mono- and dimethyl-H3K9. It has also been found to be a novel interaction partner with ER71, a transcription factor expressed in the testes of adult mice and during embryogenesis. JMJD1A is also a downstream gene of STAT3, a protein that has been found to be important in the maintenance of mouse embryonic stem (mES) cells, and decreased JMJD1A expression correlated with the differentiation of cultured mES cells following the removal of the cytokine LIF. These findings suggest that JMJD1A might be a critical signaling molecule underlying the maintenance of pluripotency in embryonic stem cells. At least two isoforms of JMJD1A are known to exist. This antibody will not cross-react with JMJD1B or JMJD1C.
Additional Names
JMJD1A,Lysine-specific demethylase 3A, KDM3A, JHDM2A, JHMD2A, TSGA
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Description
Left: Western blot analysis of JMJD1A in mouse liver tissue lysate with JMJD1A antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of JMJD1A in rat liver tissue with JMJD1A antibody at 5 μg/ml.
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Other Product Images
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Source
JMJD1A antibody was raised against a 16 amino acid peptide from near the amino terminus of human JMJD1A.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
JMJD1A antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
JMJD1A antibody can be used for detection of JMJD1A by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5365P - JMJD1A Peptide
Storage
JMJD1A antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1404 - Mouse Liver Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
20357522
Protein Accession Number
NP_060903
Short Description
Lysine-specific demethylase 3A
This product belongs to the following categories:
References- Hoog C, Schalling M, Grunder-Brundell E, et al. Analysis of a murine male germ cell-specific transcript that encodes a putative zinc finger protein. Mol. Reprod. Dev. 1991; 30:173-81.
- Sar A, Ponjevic D, Nguyen M, et al. Identification and characterization of demethylase JMJD1A as a gene upregulated in the human cellular response to hypoxia. Cell Tissue Res. 2009; 337:223-34.
- Yamane K, Toumazou C, Tsukada Y, et al. JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor. Cell 2006; 125:483-95.
- Knebel J, De Haro L, and Janknecht R. Repression of transcription by TSGA/Jmjd1a, a novel interaction partner of the ETS protein ER71. J. Cell Biochem. 2006; 99:319-29.
Datasheet 09-01W
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