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Detection Sets
Inhibitor of Apoptosis Protein Detection Set
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. This process can be interrupted by a family of proteins termed inhibitors of apoptosis (IAP). Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR). These BIRs promote protein-protein interactions with members of the TRAF family of signaling molecules as well as caspases such as caspase-3, -7, and -9, and inhibiting the apoptotic activity of these proteins. Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Other IAPs include the X-linked protein XIAP/ILP-1, the related cIAP protein which exists in two distinct isoforms, Livin (also known as KIAP as it is highly expressed in kidney), Survivin/TIAP, ILP-2, and Bruce, an extremely large IAP with ubiquitin-conjugating enzyme activity. Upregulation of these proteins inhibit the normal apoptotic process and thus may be involved in human diseases such as cancer.
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Description
For images please see PDF data sheet
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Kit Contents
cIAP Antibody, Catalog No. 3325 (50μg)
ILP-2 Antibody, Catalog No. 3017 (50μg)
Livin Antibody, Catalog No. 2505 (50μg)
NAIP Antibody, Catalog No. 3315 (50μg)
Survivin Antibody (CT), Catalog No. 2235 (50μg)
XIAP Antibody, Catalog No. 3331 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of cIAP, ILP-2, Livin, NAIP, Survivin, and XIAP by immunoblot at 1 – 2 μg/ml. These antibodies can also be used at 2 – 10 μg/ml to detect their respective proteins via immunohistochemistry / immunocytochemistry.
Tested Application
WB, IHC
Blocking Peptide
cIAP peptide (CT), Catalog No. 3325P
ILP-2 peptide (lrg), Catalog No. 3017P
Livin peptide, Catalog No. 2505P
NAIP peptide, Catalog No. 3315P
Survivin peptide, Catalog No. 2235P
XIAP peptide, Catalog No. 3331P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -20˚C.
Positive Control
- cIAP antibody: Human Lung Tissue Lysate, Catalog No. 1302
ILP-2 antibody: HepG2 Cell Lysate, Catalog No. 1211
Livin antibody: Raji Cell Lysate, Catalog No. 1207
NAIP antibody: PC-3 Cell Lysate, Catalog No. 1216
Survivin antibody: MOLT4 Cell Lysate, Catalog No. 1206
XIAP antibody: Human Kidney Tissue Lysate, Catalog No. 1305
Species Reactivity
see datasheet
Short Description
cIAP, ILP-2, Livin, NAIP, Survivin and XIAP
This product belongs to the following categories:
Related Products
References- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Verhagen AM, Coulson EJ, and Vaux DL. Inhibitor of apoptosis proteins and their relatives: IAPs and other BIRPs. Genome Biol. 2001; 2:reviews3009.1–reviews3009.10.
- Roy N, Mahadevan MS, McLean M, et al. The gene for neuronal apoptosis inhibitory protein is partially deleted in individuals with spinal muscular atrophy. Cell 1995; 80:167-78.
- Deveraux QL, Takahashi R, Savesan GS, et al. X-linked IAP is a direct inhibitor of cell-death proteases. Nature 1997; 388:300-4.
Datasheet 0803-W
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