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Primary Antibodies
IRF8 Antibody
Background
Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-α/β genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases (2,3), but IRF8 mediates activation of NF-κB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells (4,5). Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia (6).
Additional Names
IRF8
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Description
Left: Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1μg/ml in (A) the presence and (B) absence of blocking peptide.
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Source
IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy-terminus of human IRF8.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
IRF8 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human IRF8 Peptide (Cat. No. 3401P)
Application
IRF8 antibody can be used for detection of IRF8 by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3401P - IRF8 Peptide
Storage
IRF8 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1314 - Human Thymus Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
4504567
Protein Accession Number
NP_002154
Short Description
interferon regulatory factor 8
This product belongs to the following categories:
Related Products
References- Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
- Weisz A, Marx P, Sharf R, et al. The human interferon consensus sequence binding protein (H-ICSBP) is a negative regulator of enhancer elements common to interferon inducible genes. J. Biol. Chem. 1992; 267:25589-96.
- Nelson N, Marks MS, Driggers PH, et al. Interferon consensus sequence-binding protein, a member of the interferon regulatory factor family, suppresses interferon-induced gene transcription. Mol. Cell. Biol. 1993; 13:588-99.
- Tsujimura H, Tamura T, Kong HJ, et al. Toll-like receptor 9 signaling activates NF-κB through IFN regulatory factor-8/IFN consensus sequence binding protein in dendritic cells. J. Immunol. 2004; 172:6820-7.
Datasheet 09-01W
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